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KMID : 0364820090450010086
Korean Journal of Microbiology
2009 Volume.45 No. 1 p.86 ~ p.90
Identification and Distribution of the Pathogenic Microorganisms Isolated from Edible
Kim Su-Jung

Abstract
The definition of edible ice is frozen water for the use of food manufacturing, processing, or cooking, as well as
for the direct eating. It has been reported that in the process of ice manufacturing and its selling, edible ice is
contaminated with some microorganisms, which causes food poisoning and gastroenteritis. It was shown that
besides in the edible ice, germ growth caused by various reasons occurred in the mineral water, tap water, water
filtering system, and water purifier. With public awareness, in order to examine the sanitary conditions of edible
ice in the Northern area of Daegu metropolitan city, 15 places were randomly selected. As a result, 14 places
were found to be contaminated with microorganisms. After incubating on the Brain Heart Infusion (BHI) agar
plate, 80% of Gram-negative bacilli, 17% of Gram-positive cocci, and 3% of Gram-negative cocci were cultured.
Enterobacter cloacae, Chryseomonas luteola, Pantoea spp., Klebsiella pneumoniae, Acinetobacter baumannii, Acinetobacter calcoaceticus or Providencia rettgeri were detected. Gram-positive cocci cultured in BHI
agar plate from 5 specimens were identified as Staphylococcus aureus or Staphylococcus xylosus, which is well
known bacteria causing strong food poisoning. This present paper raises questions on the importance and awareness
of sanitary conditions of edible ice and the identification of pathogenic microorganisms living in the edible
ice in relation to their distribution. The examination of sanitary conditions of edible ice in other areas in Daegu
seems to be also needed to find out if there are similar cases.
KEYWORD
edible ice, E. coli, general bacteria, S. aureus
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